Exploration of the Modern Application and International Promotion of Traditional Chinese Medicine Based on the Self-Assembly Mechanism of Traditional Chinese Medicine Formulas
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Abstract
As an important part of traditional Chinese culture, traditional Chinese medicine embodies a unique theoretical system and application value. Studies have shown that the drug components in Chinese medicine formulas form complex molecular structures through self-assembly mechanisms, which can affect their pharmacodynamic properties. This study aims to explore the mechanism of self-assembly in Chinese medicine formulas, so as to promote the modernization and internationalization of Chinese medicine. In this paper, berberine-baicalin, the main component of Coptis baicalensis and Scutellaria baicalensis in traditional Chinese medicine, as an example, the differences and mechanisms of self-assembly caused by co-frying and physical mixtures are studied, and the relationship between the morphology of self-assembly and the antibacterial effect is revealed through various techniques. Studies have shown that their physical mixed forms are all nanofibers, and nanospheres in their co-decoction form can affect amino acid biosynthesis and metabolism in bacteria. These findings provide an experimental basis for the R&D and promotion of TCM formula granules, according to which we are expected to optimize the preparation method of drugs, improve efficacy, reduce adverse reactions, promote the TCM industry, and inject new vitality into its global dissemination and recognition.
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References
Zheng J, Fan R, Wu HQ, Yao HH, Yan YJ, Liu JM, et al. Directed self-assembly of herbal small molecules into sustained release hydrogels for treating neural inflammation. Nat Commun 2019; 10: 1604.
Qiao YQ, Xu YD, Liu XM, Zheng YF, Li B, Han Y, et al. Microwave assisted antibacterial action of Garcinia nanoparticles on Gram-negative bacteria. Nat Commun 2022; 13: 1−13.
Fan LL, Zhang BC, Xu AC, Shen ZC, Guo Y, Zhao RR, et al. Carrier-free, pure nanodrug formed by the self-assembly of an anticancer drug for cancer immune therapy. Mol Pharmaceut 2018; 15: 2466−2478.
Wang DL, Yu CY, Xu L, Shi LL, Tong GS, Wu JL, et al. Nucleoside Analogue-Based Supramolecular Nanodrugs Driven by Molecular Recognition for Synergistic Cancer Therapy. J Am Chem Soc 2018; 140: 8797−8806.
Huang XM, Wang PL, Li T, Tian XH, Guo WB, Xu B, et al. Self-Assemblies Based on Traditional Medicine Berberine and Cinnamic Acid for Adhesion-Induced Inhibition Multidrug-Resistant Staphylococcus aureus. ACS Appl Mater Interfaces 2020; 12: 227−237.
Tian XH, Wang PL, Li T, Huang XM, Guo WB, Yang YQ, et al. Self-assembled natural phytochemicals for synergistically antibacterial application from the enlightenment of traditional Chinese medicine combination. Acta Pharm Sin B 2019; 10: 1784−1795.
Wang PL, Guo WB, Huang GR, Zhen JH, Li YN, Li T, et al. Berberine-Based Heterogeneous Linear Supramolecules Neutralized the Acute Nephrotoxicity of Aristolochic Acid by the Self-Assembly Strategy. ACS Appl Mater Interfaces 2021; 13: 32729−32742.
Liu H, Ma ZG. Analysis on Situation of Traditional Chinese Medicine Development and Protection Strategies in China. Chin J Integr Med 2020; 26: 943−946.
Consistency evaluation between dispensing granule and traditional decoction from Coptidis Rhizoma by using an integrated quality-based strategy
Qin LL, Yu M, Zhang HX, Jia HM, Ye XC, Zou ZM. Quality markers of Baizhu dispensing granules based on multi-component qualitative and quantitative analysis combined with network pharmacology and chemometric analysi. J Ethnopharmacol 2022; 288: 114968
UPLC-QTOF-MS with chemical profiling approach for rapidly evaluating chemical consistency between traditional and dispensing granule decoctions of Tao-Hong-Si-Wu decoction
Qiu RJ, Zhang XY, Zhao C, Li M, Shang HC. Comparison of the efficacy of dispensing granules with traditional decoction: a systematic review and meta-analysis. Ann Transl Med 2018; 6: 38.
Luo H, Chen HG, Liu C, Zhang SY, Vong CT, Tan DC, et al. The key issues and development strategy of Chinese Classical Formulas pharmaceutical preparation. Chin Med 2021; 16: 1−14.
Zhuang Y, Yan JJ, Zhu W, Chen LR, Liang DH, Xu XJ. Can the aggregation be a new approach for understanding the mechanism of Traditional Chinese Medicine?. J Ethnopharmacol 2008; 117: 378−384.
Hu J, Wu ZS, Yan JJ, Pang WS, Liang DH, Xu XJ. A promising approach for understanding the mechanism of Traditional Chinese Medicine by the aggregation morphology. J Ethnopharmacol 2009; 123: 267−274.
Lv SW, Su H, Sun S, Guo YY, Liu T, Ping Y, et al. Isolation and characterization of nanometre aggregates from a bai-hu-tang decoction and their antipyretic effect. Sci Rep 2018; 8: 1−10.
Lin D, Du Q, Wang HQ, Gao GZ, Zhou JW, Ke LJ, et al. Antidiabetic micro-/nanoaggregates from ge-gen-qin-lian-tang decoction increase absorption of baicalin and cellular antioxidant activity in vitro. Biomed Res Int 2017; 2017: 9217912.
Zheng W, Sun GX, Chen JH, Li ZH, Zhang T, Wei GJ, et al. Inhibitory effects of Coptidis Rhizoma on the intestinal absorption and metabolism of Scutellariae Radix. J Ethnopharmacol 2021; 270: 113785.
Xiao SW, Liu C, Chen MJ, Zou JF, Zhang ZM, Cui X, et al. Scutellariae radix and coptidis rhizoma ameliorate glycolipid metabolism of type 2 diabetic rats by modulating gut microbiota and its metabolites. Appl Microbiol Biot 2020; 104: 303−317.
Hu ZP, Yang MY, Liu Y, Yang QY, Xie HY, Peng SH, et al. Effect of Huang-Lian Jie-Du decoction on glucose and lipid metabolism in type 2 diabetes mellitus: A systematic review and meta-analysis. Front Pharmacol 2021; 12: 648861.
Zhang ZW, Zhang NN, Li YM. Content Change of Main Chemical Composition Before and After Compatibility of Scutellaria baicalensis and Coptis chinensis. Chin J Exp Tradit Med Form 2012; 18: 58−62.
Wang JR, Tanaka T, Zhang H, Kouno I, Jiang Z H. Formation and conformation of baicalin–berberine and wogonoside–berberine complexes. Chem Pharm Bull 2012; 60: 706−711.
Li W, Song FR, Liu ZQ, Liu SY. Studies on change of chemical composition in Coptis-Scute herb couple by using HPCE and HPLC. Acta Pharm Sin 2008; 43: 191−194.
Li T, Wang PL, Guo WB, Huang XM, Tian XH, Wu GR, et al. Natural Berberine-Based Chinese Herb Medicine Assembled Nanostructures with Modified Antibacterial Application. ACS Nano 2019; 13: 6770−6781.
Cai XX, Weng QX, Lin JM, Chen GQ, Wang SY. Radix Pseudostellariae protein-curcumin nanocomplex: improvement on the stability, cellular uptake and antioxidant activity of curcumin. Food Chem Toxicol 2021; 151: 112110.
Bag BG, Dash SS. Hierarchical self-assembly of a renewable nanosized pentacyclic dihydroxy-triterpenoid betulin yielding flower-like architectures. Langmuir 2015; 31: 13664−13672.
Kumorek M, Minisy IM, Krunclová T, Voršiláková M, Venclíková K, Chánová EM, et al. pH-responsive and antibacterial properties of self-assembled multilayer films based on chitosan and tannic acid. Mater Sci Eng C 2020; 109: 110493.
Li Qk, Li JZ, Yu WK, Wang ZH, Li JW, Feng XJ, et al. De novo design of a pH-triggered self-assembled β-hairpin nanopeptide with the dual biological functions for antibacterial and entrapment. J Nanobiotechnol 2021; 19: 1−19.
Naskar J, Banerjee A. Concentration dependent transformation of oligopeptide based nanovesicles to nanotubes and an application of nanovesicles. Chem–Asian J 2009; 4: 1817−1823.
Liu J, Kim YT, Kwon YU. Hematite Thin Films with Various Nanoscopic Morphologies Through Control of Self-Assembly Structures. Nanoscale Res Lett 2015; 10: 1−10.
Von Staszewski M, Ruiz-Henestrosa VMP, Pilosof AMR. Green tea polyphenols-β-lactoglobulin nanocomplexes: Interfacial behavior, emulsification and oxidation stability of fish oil. Food Hydrocoll 2014; 35: 505−511.
Gao M, Yang YF, Bergfel A, Huang LL, Zheng L, Bowden TM. Self-assembly of cholesterol end-capped polymer micelles for controlled drug delivery. J Nanobiotechnol 2020; 18: 1−10.
Zhang M, Ye LL, Huang H, Cheng DD, Liu KX, Wu WB, et al. Micelles self-assembled by 3-O-β-d-glucopyranosyl latycodigenin enhance cell membrane permeability, promote antibiotic pulmonary targeting and improve anti-infective efficacy. J Nanobiotechnol 2020; 18: 1−15.
Wang YM, Wang J, Wang TS, Xu YS, Shi L, Wu YT, et al. Pod-Like Supramicelles with Multicompartment Hydrophobic Cores Prepared by Self-Assembly of Modified Chitosan. Nano-micro Lett 2016; 8: 151−156.
Zhai Y, Chai W, Cao WW, Sun ZP, Huang YD. Organogelators based on p-alkoxylbenzamide and their self-assembling properties. Front Chem Sci Eng 2015; 9: 488−493.
Ghosh D, Farahani AD, Martin AD, Thordarson P, Damodaran KK. Unraveling the Self-Assembly Modes in Multicomponent Supramolecular Gels Using Single-Crystal X-ray Diffraction. Chem Mater 2020; 32: 3517−3527.
Smulders MMJ, Schenning APHJ, Meijer EW. Insight into the mechanisms of cooperative self-assembly: The “sergeants-and-soldiers” principle of chiral and achiral C3-symmetrical discotic triamides. J Am Chem Soc 2008; 130: 606−611.
Roqanian S, Meratan AA, Ahmadian S, Shafizadeh M, Ghasemi A, Karami L. Polyphenols protect mitochondrial membrane against permeabilization induced by HEWL oligomers: Possible mechanism of action. Int J Biol Macromol 2017; 103: 709−720.
Guo NH, Wang CL, Shang C, You X, Zhang LY, Liu WB. Integrated study of the mechanism of tyrosinase inhibition by baicalein using kinetic, multispectroscopic and computational simulation analyses. Int J Biol Macromol 2018; 118: 57−68.
Gao YX, Hao J, Wu JD, Li Y, Lin Y, Hu J, et al. Cooperative supramolecular helical assembly of a pyridinium-tailored methyl glycyrrhetate. Soft Matter 2016; 12: 8979−8982.
Zhu GJ, Cheng SW, Hu Y, Li W, Mu JS. Synthesis and crystallization-driven solution self-assembly of PE-b-PMMA: controlling Micellar morphology through crystallization temperature and molar mass. J Polym Res 2020; 27: 1−11.
Huang RL, Wang YF, Qi W, Su RX, He ZM. Temperature-induced reversible self-assembly of diphenylalanine peptide and the structural transition from organogel to crystalline nanowires. Nanoscale rRes Lett 2014; 9: 1−9.
Jin J, Yang F, Li B, Liu D, Wu LH, Li Y, et al. Temperature-regulated self-assembly of lipids at free bubbles interface: A green and simple method to prepare micro/nano bubbles. Nano Res 2020; 13: 999−1007.
Frassinetti S, Falleni A, Del Carratore R. Effect of itraconazole on Staphylococcus aureus biofilm and extracellular vesicles formation. Microb Pathogenesis 2020; 147: 104267.
Tang JN, Kang MS, Chen HC, Shi XM, Zhou R, Chen J, et al. The staphylococcal nuclease prevents biofilm formation in Staphylococcus aureus and other biofilm-forming bacteria. Sci China Life Sci 2011; 54: 863−869.
Zhang JM, Liao W, He YX, He Y, Yan D, Fu CM. Study on intestinal absorption and pharmacokinetic characterization of diester diterpenoid alkaloids in precipitation derived from Fuzi-Gancao herb-pair decoction for its potential interaction mechanism investigation. J Ethnopharmacol 2013; 147: 128−135.
Yan R, Wang Y, Shen WJ, Liu YP, Di X. Comparative pharmacokinetics of dehydroevodiamine and coptisine in rat plasma after oral administration of single herbs and Zuojinwan prescription. Fitoterapia 2011; 82: 1152−1159.
Zhan JYX, Zheng KYZ, Zhang WL, Chen JP, Yao P, Bi CWC, et al. Identification of Angelica oil as a suppressor for the biological properties of Danggui Buxue tang: A Chinese herbal decoction composes of Astragali Radix and Angelica Sinensis Radix. J Ethnopharmacol 2014; 154: 825−831.